Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.18/2835
Título: An integrative assessment to determine the genotoxic hazard of estuarine sediments: combining cell and whole-organism responses
Autor: Costa, Pedro Manuel
Pinto, Miguel
Vicente, Ana M.
Gonçalves, Cátia
Rodrigo, Ana P.
Louro, Henriqueta
Costa, Maria Helena
Caeiro, Sandra
Silva, Maria João
Palavras-chave: Genotoxicidade Ambiental
Comet Assay
Environmental Risk Assessment
HepG2 Cells
Oxidative Stress
Sediment Contamination
Data: 10-Dez-2014
Editora: Frontiers
Citação: Front Genet. 2014 Dec 10;5:437. doi: 10.3389/fgene.2014.00437. eCollection 2014
Resumo: The application of the Comet assay in environmental monitoring remains challenging in face of the complexity of environmental stressors,e.g.,when dealing with estuarine sediments,that hampers the drawing of cause-effect relationships. Although the in vitro The application of the Comet assay in environmental monitoring remains challenging in face of the complexity of environmental stressors, e.g., when dealing with estuarine sediments, that hampers the drawing of cause-effect relationships. Although the in vitro Comet assay may circumvent confounding factors, its application in environmental risk assessment (ERA) still needs validation. As such, the present work aims at integrating genotoxicity and oxidative DNA damage induced by sediment-bound toxicants in HepG2 cells with oxidative stress-related effects observed in three species collected from an impacted estuary. Distinct patterns were observed in cells exposed to crude mixtures of sediment contaminants from the urban/industrial area comparatively to the ones from the rural/riverine area of the estuary, with respect to oxidative DNA damage and oxidative DNA damage. The extracts obtained with the most polar solvent and the crude extracts caused the most significant oxidative DNA damage in HepG2 cells, as measured by the formamidopyrimidine-DNA glycosylase (FPG)-modified Comet assay. This observation suggests that metals and unknown toxicants more hydrophilic than polycyclic aromatic hydrocarbons may be important causative agents, especially in samples from the rural part of the estuary, where oxidative DNA damage was the most significant. Clams, sole, and cuttlefish responded differentially to environmental agents triggering oxidative stress, albeit yielding results accordant with the oxidative DNA damage observed in HepG2 cells. Overall, the integration of in vivo biomarker responses and Comet assay data in HepG2 cells yielded a comparable pattern, indicating that the in vitro FPG-modified Comet assay may be an effective and complementary line-of-evidence in ERA even in particularly challenging, natural, scenarios such as estuarine environments.
Descrição: Creative Commons Attribution License
Peer review: yes
URI: http://hdl.handle.net/10400.18/2835
DOI: 10.3389/fgene.2014.00437
ISSN: 1664-8021
Versão do Editor: http://journal.frontiersin.org/journal/10.3389/fgene.2014.00437/abstract
Aparece nas colecções:DGH - Artigos em revistas internacionais

Ficheiros deste registo:
Ficheiro Descrição TamanhoFormato 
Costa2014_frontiers.pdf1,62 MBAdobe PDFVer/Abrir


FacebookTwitterDeliciousLinkedInDiggGoogle BookmarksMySpace
Formato BibTex MendeleyEndnote Degois 

Todos os registos no repositório estão protegidos por leis de copyright, com todos os direitos reservados.