Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.18/2768
Título: Neuroprotective effect of steroidal alkaloids on glutamate-induced toxicity by preserving mitochondrial membrane potential and reducing oxidative stress
Autor: Taveira, M.
Sousa, C.
Valentão, P.
Ferreres, F.
Teixeira, João Paulo
Andrade, P.B.
Palavras-chave: Steroidal Alkaloids
Neuroprotection
Oxidative Stress
Mitochondrial Membrane Potential
Cholinergic Receptors
Genotoxidade Ambiental
Data: Mar-2014
Editora: Elsevier
Citação: J Steroid Biochem Mol Biol. 2014 Mar;140:106-15. doi: 10.1016/j.jsbmb.2013.12.013. Epub 2013 Dec 24.
Resumo: Several evidences suggest that enhanced oxidative stress is involved in the pathogenesis and/or progression of several neurodegenerative diseases. The aim of this study was to investigate for the first time whether both extracts from tomato plant (Lycopersicon esculentum Mill.) leaves and their isolated steroidal alkaloids (tomatine and tomatidine) afford neuroprotective effect against glutamate-induced toxicity in SH-SY5Y neuroblastoma cells and to elucidate the mechanisms underlying this protection. Steroidal alkaloids from tomato are well known for their cholinesterases' inhibitory capacity and the results showed that both purified extracts and isolated compounds, at non-toxic concentrations for gastric (AGS), intestinal (Caco-2) and neuronal (SH-SY5Y) cells, have the capacity to preserve mitochondria membrane potential and to decrease reactive oxygen species levels of SH-SY5Y glutamate-insulted cells. Moreover, the use of specific antagonists of cholinergic receptors allowed observing that tomatine and tomatidine can interact with nicotinic receptors, specifically with the α7 type. No effect on muscarinic receptors was noticed. In addition to the selective cholinesterases' inhibition revealed by the compounds/extracts, these results provide novel and important insights into their neuroprotective mechanism. This work also demystifies the applicability of these compounds in therapeutics, by demonstrating that their toxicity was overestimated for long time.
Peer review: yes
URI: http://hdl.handle.net/10400.18/2768
DOI: 10.1016/j.jsbmb.2013.12.013
ISSN: 0960-0760
Versão do Editor: http://www.sciencedirect.com/science/article/pii/S0960076013002860
Aparece nas colecções:DSA - Artigos em revistas internacionais

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