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|Título:||Variation of DNA damage levels in peripheral blood mononuclear cells isolated in different laboratories|
Teixeira, João Paulo
van Schooten, F.J.
López de Cerain, A.
Environmental Cancer Risk
Ar e Saúde Ocupacional
|Editora:||Oxford University Press/UK Environmental Mutagen Society|
|Citação:||Mutagenesis. 2014 Jul;29(4):241-9. doi: 10.1093/mutage/geu012. Epub 2014 Apr 15.|
|Resumo:||This study investigated the levels of DNA strand breaks and formamidopyrimidine DNA glycosylase (FPG) sensitive sites, as assessed by the comet assay, in peripheral blood mononuclear cells (PBMC) from healthy women from five different countries in Europe. The laboratory in each country (referred to as 'centre') collected and cryopreserved PBMC samples from three donors, using a standardised cell isolation protocol. The samples were analysed in 13 different laboratories for DNA damage, which is measured by the comet assay. The study aim was to assess variation in DNA damage in PBMC samples that were collected in the same way and processed using the same blood isolation procedure. The inter-laboratory variation was the prominent contributor to the overall variation. The inter-laboratory coefficient of variation decreased for both DNA strand breaks (from 68 to 26%) and FPG sensitive sites (from 57 to 12%) by standardisation of the primary comet assay endpoint with calibration curve samples. The level of DNA strand breaks in the samples from two of the centres (0.56-0.61 lesions/10(6) bp) was significantly higher compared with the other three centres (0.41-0.45 lesions/10(6) bp). In contrast, there was no difference between the levels of FPG sensitive sites in PBMC samples from healthy donors in the different centres (0.41-0.52 lesion/10(6) bp).|
|Versão do Editor:||http://mutage.oxfordjournals.org/content/29/4/241.abstract|
|Aparece nas colecções:||DSA - Artigos em revistas internacionais|
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|2014 Mutagenesis Moller WP3.pdf||352,42 kB||Adobe PDF||Ver/Abrir Acesso Restrito. Solicitar cópia ao autor!|
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