Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.18/2747
Título: Comet assay reveals no genotoxicity risk of cationic solid lipid nanoparticles.
Autor: Doktorovova, S.
Silva, A.M.
Gaivão, I.
Souto, E.B.
Teixeira, João Paulo
Martins-Lopes, P.
Palavras-chave: Comet Assay
Caco-2
HepG2
Genotoxicity
Solid Lipid Nanoparticles
Ar e Saúde Ocupacional
Genotoxidade Ambiental e Ocupacional
Data: Abr-2014
Editora: John Wiley & Sons, Ltd
Citação: J Appl Toxicol. 2014 Apr;34(4):395-403. doi: 10.1002/jat.2961. Epub 2013 Nov 15.
Resumo: Cationic solid lipid nanoparticles (cSLN) are colloidal carriers for genes or drugs, particularly lipophilic drugs. Several reports exist on their high efficiency, but only a few studies report the effect of cSLNs on living cells. In the present work, internalization, cell viability (alamar blue assay) and genotoxic potential (alkaline comet assay) of three cSLN formulations (A–C) were evaluated in HepG2 and Caco-2 cells. cSLN showed an average hydrodynamic diameter (z-ave) of 141–222 nm, zeta-potential of 55.0–72.5mV and polidispersity indices (PdI) of 0.336–0.421. Dispersion in physiological buffers increased z-ave and PdI. 0.01mgml–1 cSLN unaffected cell viability, but 1.0mgml–1 significantly decreased it, being cSLN-C (Compritol-based) the most toxic and HepG2 the most affected. DNA damage was not significantly increased by 0.1mgml–1 cSLN but damage was observed at 1.0mgml–1 cSLN-C. Thus, no genotoxicity is to be expected at concentrations that do not reduce cell viability.
Peer review: yes
URI: http://hdl.handle.net/10400.18/2747
DOI: 10.1002/jat.2961
ISSN: 0260-437X
Versão do Editor: http://onlinelibrary.wiley.com/doi/10.1002/jat.2961/abstract
Aparece nas colecções:DSA - Artigos em revistas internacionais

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