Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.18/2697
Título: Applicability of In Vitro Methods to Study Patulin Bioaccessibility and Its Effects on Intestinal Membrane Integrity
Autor: Assunção, Ricardo
Ferreira, Mariana
Martins, Carla
Diaz, Irene
Padilla, Beatriz
Dupond, Didier
Bragança, Mauro
Alvito, Paula
Palavras-chave: Segurança Alimentar
Toxicologia
Modelos de Digestão in vitro
Data: 29-Jul-2014
Editora: Taylor & Francis: STM, Behavioural Science and Public Health Titles
Citação: J Toxicol Environ Health A. 2014;77(14-16):983-92. doi: 10.1080/15287394.2014.911138
Resumo: In human health risk assessment, ingestion of food is considered a major route of exposure to many contaminants, although the total amount of an ingested contaminant (external dose) does not always reflect the quantity available for the body (internal dose). In this study, two in vitro methods were applied to study bioaccessibility and intestinal membrane integrity of cells exposed to patulin, a mycotoxin with significant public health risk. Seven artificially contaminated fruit juices were assayed in the presence or absence of a standard meal, showing a significant difference for bioaccessibility values between contaminated samples alone (mean 27.65 ± 13.50%) and combinations with a standard meal (mean 7.89 ± 4.03%). Different concentrations of patulin (PAT) and cysteine (CYS) (protector agent) were assayed in Caco-2 cells monolayers. At 95 μM, PAT produced a marked decrease in transepithelial electrical resistance (TEER). This effect was significantly reduced when 400 μM and 4000 μM CYS was added to the cells. Combined use of in vitro digestion models with other techniques using intestinal cell lines, such as in vitro intestinal absorption models that use Caco-2 cells, may offer a more comprehensive model of what is occurring during digestion and absorption processes. The study of beneficial effects of protective agents would also be enhanced.
Peer review: yes
URI: http://hdl.handle.net/10400.18/2697
DOI: 10.1080/15287394.2014.911138
ISSN: 1528-7394
Versão do Editor: http://www.tandfonline.com/doi/full/10.1080/15287394.2014.911138#tabModule
Aparece nas colecções:DAN - Artigos em revistas internacionais

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