Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.18/2024
Título: Influence of the surface coating on the cytotoxicity, genotoxicity and uptake of gold nanoparticles in human HepG2 cells
Autor: Fraga, S.
Faria, H.
Soares, M.E.
Duarte, J.A.
Soares, L.
Pereira, E.
Costa-Pereira, C.
Teixeira, João Paulo
de Lourdes Bastos, M.
Carmo, H.
Palavras-chave: DNA Damage
HepG2 Cells
Cellular Uptake
Cytotoxicity
Gold Nanoparticles
Surface Properties
Ar e Saúde Ocupacional
Data: 25-Mar-2013
Editora: John Wiley & Sons
Citação: J Appl Toxicol. 2013 Oct;33(10):1111-9. Epub 2013 Mar 25
Resumo: The toxicological profile of gold nanoparticles (AuNPs) remains controversial. Significant efforts to develop surface coatings to improve biocompatibility have been carried out. In vivo biodistribution studies have shown that the liver is a target for AuNPs accumulation. Therefore, we investigated the effects induced by ~20 nm spherical AuNPs (0-200 μM Au) with two surface coatings, citrate (Cit) compared with 11-mercaptoundecanoic acid (11-MUA), in human liver HepG2 cells. Cytotoxicity was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reduction and lactate dehydrogenase (LDH) release assays after 24 to 72 h of incubation. DNA damage was assessed by the comet assay, 24 h after incubation with the capped AuNPs. Uptake and subcellular distribution of the tested AuNPs was evaluated by quantifying the gold intracellular content by graphite furnace atomic absorption spectrometry (GFAAS) and transmission electron microscopy (TEM), respectively. The obtained results indicate that both differently coated AuNPs did not induce significant cytotoxicity. An inverse concentration-dependent increase in comet tail intensity and tail moment was observed in Cit-AuNPs- but not in MUA-AuNPs-exposed cells. Both AuNPs were internalized in a concentration-dependent manner. However, no differences were found in the extent of the internalization between the two types of NPs. Electron-dense deposits of agglomerates of Cit- and MUA-AuNPs were observed either inside endosomes or in the intercellular spaces. In spite of the absence of cytotoxicity, DNA damage was observed after exposure to the lower concentrations of Cit- but not to MUA-AuNPs. Thus, our data supports the importance of the surface properties to increase the biocompatibility and safety of AuNPs.
Peer review: yes
URI: http://hdl.handle.net/10400.18/2024
ISSN: 0260-437X
Versão do Editor: http://onlinelibrary.wiley.com/doi/10.1002/jat.2865/full
Aparece nas colecções:DSA - Artigos em revistas internacionais

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