Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.18/1972
Título: Genotoxicity evaluation of nanosized titanium dioxide, synthetic amorphous silica and multi-walled carbon nanotubes in human lymphocytes
Autor: Tavares, Ana Maria
Louro, Henriqueta
Antunes, Susana
Quarré, S.
Simar, S.
Temmerman, P.D.
Verleysen, E
Mast, J.
Jensen, K.A.
Norppa, H.
Nesslany, F.
Silva, Maria João
Palavras-chave: Genotoxicidade Ambiental
Nanomaterials
Genotoxicity
Human Cells
Titanium Dioxide
Carbon Nanotubes
Synthetic amorphous silica nanomaterials
Multiwalled carbon nanotubes
Micronucleus assay
Primary human lymphocytes
Morphology and size
Data: 27-Jun-2013
Editora: Elsevier/ European Society for Toxicology in Vitro
Citação: Toxicol In Vitro. 2014 Feb;28(1):60-9.Epub 2013 Jun 27
Resumo: Toxicological characterization of manufactured nanomaterials (NMs) is essential for safety assessment, while keeping pace with innovation from their development and application in consumer products. The specific physicochemical properties of NMs, including size and morphology, might influence their toxicity and have impact on human health. The present work aimed to evaluate the genotoxicity of nanosized titanium dioxide (TiO2), synthetic amorphous silica (SAS) and multiwalled carbon nanotubes (MWCNT), in human lymphocytes. The morphology and size of those NMs were characterized by transmission electron microscopy, while the hydrodynamic particle size-distributions were determined by dynamic light scattering. Using a standardized procedure to ensure the dispersion of the NMs and the cytokinesis-block micronucleus assay (without metabolic activation), we observed significant increases in the frequencies of micronucleated binucleated cells (MNBC) for some TiO2 NMs and for two MWCNTs, although no clear dose–response relationships could be disclosed. In contrast, all forms of SAS analyzed in this study were unable to induce micronuclei. The present findings increase the weight of evidence towards a genotoxic effect of some forms of TiO2 and some MWCNT. Regarding safety assessment, the differential genotoxicity observed for closely related NMs highlights the importance of investigating the toxic potential of each NM individually, instead of assuming a common mechanism and equal genotoxic effects for a set of similar NMs.
Peer review: yes
URI: http://hdl.handle.net/10400.18/1972
ISSN: 0887-2333
Versão do Editor: http://www.sciencedirect.com/science/article/pii/S0887233313001604
Aparece nas colecções:DGH - Artigos em revistas internacionais



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