Repositório Científico do Instituto Nacional de Saúde >
Departamento de Doenças Infecciosas >
DDI - Artigos em revistas internacionais >

Please use this identifier to cite or link to this item: http://hdl.handle.net/10400.18/197

Title: Serotyping of naturally Toxoplasma gondii infected meat-producing animals
Authors: Sousa, Susana
Canada, Nuno
Costa, José Manuel Correia da
Dardé, Marie-Laure
Keywords: Toxoplasma gondii
Serotyping
Meat-producing-animals
Infecções Sistémicas e Zoonoses
Issue Date: 19-Apr-2010
Publisher: Elsevier
Citation: Vet Parasitol. 2010 Apr 19;169(1-2):24-8. Epub 2009 Dec 28
Abstract: Serotyping was previously described as a promising method for typing strains of Toxoplasma gondii. The majority of precedent studies utilized serum samples collected from human patients with different T. gondii-associated pathologies. The aim of this work was to study the applicability of the same procedure for serotyping naturally infected meat-producing animals. An ELISA test based on GRA6 and GRA7 C-terminal polymorphic peptides was used. Peptide GRA6II has polymorphisms specific for the archetypal strains type II, GRA6I/III for strains type I and III, GRA7I for strains type I and GRA7III for strains type III. As reference material, and to validate this approach, serum samples from eleven free-range chickens and fifteen pigs used for Toxoplasma genotypes isolation were selected. These strains integrate the Biological Resource Centre (BRC) ToxoBS Bank. Three serum samples from chickens and two from pigs had serotyping results in agreement with genotyping. Thirty-five serum samples from chickens, twenty-nine from pigs and fifty from sheep, seropositive for T. gondii, from which no isolate was obtained, were also serotyped. Serotype III appeared significantly more frequent among sheep. Our results show that serotyping still need refinement, but may become a valuable tool for typing Toxoplasma strains from animal origin.
Peer Reviewed: yes
URI: http://hdl.handle.net/10400.18/197
ISSN: 0304-4017
Publisher version: http://www.sciencedirect.com/science/article/pii/S0304401709007481
Appears in Collections:DDI - Artigos em revistas internacionais

Files in This Item:

File Description SizeFormat
Serotyping of naturally Toxoplasma gondii infected meat-producing animals.pdf140.77 kBAdobe PDFView/Open
Restrict Access. You can request a copy!
Statistics
FacebookTwitterDeliciousLinkedInDiggGoogle BookmarksMySpaceOrkut
Formato BibTex mendeley Endnote Logotipo do DeGóis 

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

 

  © 2010 www.insa.pt - Todos os direitos reservados | Feedback Ministério da Saúde

Estamos no RCAAP Governo Português separator Ministério da Educação e Ciência   Fundação para a Ciência e a Tecnologia

Financiado por:

POS_C UE