Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.18/1914
Título: Validation of A HPLC-UV Method for the Simultaneous Determination of Acesulfame K, Saccharin And Aspartame in Tabletop Intense Sweeteners
Autor: Veiga, Cláudia
Serra, Celeste
Vasco, Elsa
Palavras-chave: Composição de Alimentos
HPLC
Adoçantes de Mesa
Acesulfame k
Sacarina
Aspartame
Data: Ago-2013
Editora: Instituto Nacional de Saúde Doutor Ricardo Jorge, IP
Resumo: Introduction Arti'cial sweeteners are substances of low calori'c value, used as food additives to give a!sweet taste to foodstu(s, reducing the energy supplied by them. It is well known that the increase of sugar consumption is related with two severe health problems diabetes and obesity. In this way, arti'cial sweeteners are normally used in dietetic products and o)en present in so) drinks and tabletop intense sweeteners, among others products. In order to obtain consistent, reliable and accurate data it is important to use validated methods. Only in this way, obtained occurrence results could be used in exposure studies to evaluate the intake of arti'cial sweeteners by Portuguese population. In this study a!method of high performance liquid chromatography with ultraviolet detection (HPLC- UV) for simultaneous determination of acesulfame k, saccharin and aspartame in tabletop intense sweeteners was developed and validated. Method validation was performed in order to obtain accreditation by ISO 17025. Experimental Method /e analytical method, based on EN 12856, was performed in a!PrevailTM C18 (250 x 4.6mm, 5 μm) column, with the mobile phase phosphate bu(er:acetonitrile (95:5 V/V) and a!=ow rate of 1 ml/min. Detection wavelength was 220 nm for all the sweeteners. Identi'cation was carried out on the basis of the retention time and quantita- tive determination by external standard method. /e method validation included the evaluation of calibration curve parameters, limits, repeatability, precision and accuracy according to the criteria established by ISO 8466 and ISO 5725. /e precision study was performed with tabletop intense sweeteners in granulated, liquid and tablet form, which were purchased from supermarkets in Lisbon city, Portugal, between October and November 2012.Results /e method was validated in a!working range of 4-40 μg/mL for acesulfame k and saccharin and 3.6-36 μg/mL for aspartame. /e values obtained for the limits of detection (LOD) and quanti'cation (LOQ) were 1-4 μg/mL (0.1-0.4 g/kg of sample) for acesulfame k, 0.3-4 μg/mL (0.03-0.4 g/kg of sample) for saccharin and 0.5-3.6 μg/mL (0.05-0.36 g/kg of sample) for aspartame, respectively. /e repeatability (RSDr%) and the precision (RSDR%) ranged from 0.95 to 4.33 and from 1.71 to 5.21, respectively. /e absolute z-scores ob- tained in accuracy evaluation was <2. Conclusion /e method proposed in this work allows the simultaneous quanti'cation of acesulfame k, saccharin and aspartame and proved to be accurate for the determination of these additives in tabletop intense sweeteners.
Descrição: Abstract publicado em: Livro de abstracts do EuroAnalisys 2013 (Abstract ID 0215)
Peer review: yes
URI: http://hdl.handle.net/10400.18/1914
Aparece nas colecções:DAN - Posters/abstracts em congressos internacionais

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