Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.18/185
Título: Coinfections of Rickettsia slovaca and Rickettsia helvetica with Borrelia lusitaniae in ticks collected in a Safari Park, Portugal
Autor: Milhano, Natacha
Carvalho, Isabel Lopes de
Alves, Ana Sofia
Arroube, Sofia
Soares, Jorge
Rodriguez, Pablo
Carolino, Manuela
Núncio, Maria Sofia
Piesman, Joseph
Sousa, Rita de
Palavras-chave: Ticks
Rickettsia
Borrelia
Safari park
Portugal
Estudos de Vectores e Doenças Infecciosas
Data: Dez-2010
Editora: Elsevier
Citação: Ticks Tick Borne Dis. 2010 Dec;1(4):172-7
Resumo: Borrelia and Rickettsia bacteria are the most important tick-borne agents causing disease in Portugal. Identification and characterization of these circulating agents, mainly in recreational areas, is crucial for the development of preventive measures in response to the gradually increasing exposure of humans to tick vectors. A total of 677 questing ticks including Dermacentor marginatus, Rhipicephalus sanguineus, Ixodes ricinus, Hyalomma lusitanicum, H. marginatum, and Haemaphysalis punctata were collected in a Safari Park in Alentejo, Portugal, to investigate the prevalences of infection and characterize Borrelia and Rickettsia species. From a total of 371 ticks tested by PCR for Borrelia burgdorferi sensu lato (s.l.), of which 247 were tested for Rickettsia, an infection prevalence of 18.3% was found for B. lusitaniae and 55.1% for Rickettsia spp. Sequence analysis of positive amplicons identified the presence of B. lusitaniae (18.3%), R. monacensis strain IRS3 (51.7%), and R. helvetica (48.3%) in I. ricinus. R. slovaca (41.5%), R. raoultii (58.5%), and also B. lusitaniae (21%) were identified in D. marginatus ticks. One (5.9%) H. lusitanicum was infected with B. lusitaniae, and R. massiliae was found in one Rhipicephalus sanguineus. Coinfection was found in 7 (20%) I. ricinus and 34 (23.3%) D. marginatus ticks. We report, for the first time, simultaneous infection with R. helvetica and B. lusitaniae and also R. slovaca, the agent of TIBOLA/DEBONEL, with B. lusitaniae. Additionally, 6 isolates of B. lusitaniae were established, and isolates of Rickettsia were also obtained for the detected species using tick macerates cultured in mammalian and mosquito cell lines. This report describes the detection and isolation of tick-borne agents from a Portuguese Safari Park, highlighting the increased likelihood of infection with multiple agents to potential visitors or staff.
Peer review: yes
URI: http://hdl.handle.net/10400.18/185
ISSN: 1877-959X
Versão do Editor: http://www.sciencedirect.com/science/article/pii/S1877959X10000713
Aparece nas colecções:DDI - Artigos em revistas internacionais

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