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Title: Genome diversity in the genera Fructobacillus, Leuconostoc and Weissella determined by physical and genetic mapping
Author: Chelo, Ivo M.
Zé-Zé, Líbia
Tenreiro, Rogério
Keywords: Estudos de Vectores e Doenças Infecciosas
Issue Date: Feb-2010
Publisher: Society for General Microbiology
Citation: Microbiology. 2010 Feb;156(Pt 2):420-30. Epub 2009 Nov 5
Abstract: Pulsed-field gel electrophoresis analysis of chromosomal single and double restriction profiles of 17 strains belonging to three genera of ‘Leuconostocaceae’ was done, resulting in physical and genetic maps for three Fructobacillus, six Leuconostoc and four Weissella strains. AscI, I-CeuI, NotI and SfiI restriction enzymes were used together with Southern hybridization of selected probes to provide an assessment of genomic organization in different species. Estimated genome sizes varied from 1408 kb to 1547 kb in Fructobacillus, from 1644 kb to 2133 kb in Leuconostoc and from 1371 kb to 2197 kb in Weissella. Other genomic characteristics of interest were analysed, such as oriC and terC localization and rrn operon organization. The latter seems markedly different in Weissella, in both number and disposition in the chromosome. Comparisons of intra- and intergeneric features are discussed in the light of chromosome rearrangements and genomic evolution.
Peer review: yes
ISSN: 1350-0872
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Appears in Collections:DDI - Artigos em revistas internacionais

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