Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.18/1758
Título: Cytotoxic and genotoxic potential of sediments from the Portuguese Mira-River Estuary
Autor: Pinto, Miguel
Louro, Henriqueta
Costa, Pedro Manuel
Costa, Maria Helena
Caeiro, Sandra
Lavinha, João
Silva, Maria João
Palavras-chave: Genotoxicidade Ambiental
Estuary Sediments
Genotoxicity
Comet Assay
Data: 3-Mar-2013
Editora: Instituto Nacional de Saúde Doutor Ricardo Jorge, IP
Resumo: The Mira-River Estuary (SW Portugal) although being a protected natural area has been affected by urban pollution from the village of Vila Nova de Milfontes (VNMF) and by contaminants from farming and aquaculture activities. The present study is part of a broader project comprising the evaluation of environmental and human health risks from potentially contaminated estuaries and aims to assess the cytotoxic and genotoxic potential of sediments from the Mira-River Estuary. Sediments were collected from two sites: Mf located near VNMF, and M near an aquaculture discharge channel. Organic and inorganic contaminants were extracted as four fractions using: methanol:dichloromethane (F1), dichloromethane (F2), hexane (F3) and methanol (F4) and recovered in DMSO. Cytotoxicity and genotoxicity were evaluated through the neutral red assay and the comet (coupled with DNA repair endonucleases) and micronucleus assays, respectively, in the human hepatoma-derived (HepG2) cell line. Contaminants analysis was carried out by GC-MS and ICP-MS for organic and metallic contaminants, respectively. A dose-related decrease in cell viability indicating cytotoxicity was observed for sample M, extracts F1, F2 and F4. A significant increase in the level of DNA and chromosome damage was observed for sub-cytotoxic doses of extracts F1, F2 and F3, by the comet and micronucleus assays, respectively. Additionally, oxidative DNA damage was also detected in the same samples. All Mf fractions failed to induce significant cytotoxic or genotoxic effects. These data agree with contaminants characterisation, given that a higher level of known genotoxic metals and PAHs were found in sample M but not in Mf. Overall, our results suggest that urban pollution of Mira-River Estuary sediments might be less hazardous than contamination from the aquaculture and agriculture activities, raising the question of the safe consumption of aquatic species grown in aquacultures, and of aquacultures as potential sources of aquatic pollution.
URI: http://hdl.handle.net/10400.18/1758
Aparece nas colecções:DGH - Posters/abstracts em congressos internacionais

Ficheiros deste registo:
Ficheiro Descrição TamanhoFormato 
Poster Boston 2013 HERA.pdf713,94 kBAdobe PDFVer/Abrir


FacebookTwitterDeliciousLinkedInDiggGoogle BookmarksMySpace
Formato BibTex MendeleyEndnote Degois 

Todos os registos no repositório estão protegidos por leis de copyright, com todos os direitos reservados.