Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.18/1735
Título: Effects of occupational exposure to tobacco smoke: is there a link between environmental exposure and disease?
Autor: Pacheco, Solange A.
Torres, Vukosava M.
Louro, Henriqueta
Gomes, Filomena
Lopes, Carlos
Marçal, Nelson
Fragoso, Elsa
Martins, Carla
Oliveira, Cátia L.
Hagenfeldt, Manuela
Bugalho-Almeida, António
Penque, Deborah
Simões, Tânia
Palavras-chave: Genómica Funcional e Estrutural
Fumo de Tabaco
Genotoxicidade Ambiental
Avaliação do Risco
Proteomica
Lisboa
Portugal
Environmental Tobbaco Smoke
Health Effects
Occupational Exposure
Respiratory Diseases
Data: Mar-2013
Editora: Taylor & Francis: STM, Behavioural Science and Public Health Titles
Citação: J Toxicol Environ Health A. 2013;76(4-5):311-27. doi: 10.1080/15287394.2013.757269
Resumo: In a previous study, evidence was provided that indoor secondhand tobacco smoke (SHS) air pollution remains high in Lisbon restaurants where smoking is allowed, regardless of the protective measures used. The aim of this study was to determine in these locations the levels of polycyclic aromatic hydrocarbons (PAH) associated with the particulate phase of SHS (PPAH), a fraction that contains recognized carginogens, such as benzo[a]pyrene (BaP). Data showed that restaurant smoking areas might contain PPAH levels as high as 110 ng/m(3), a value significantly higher than that estimated for nonsmoking areas (30 ng/m(3)) or smoke-free restaurants (22 ng/m(3)). The effective exposure to SHS components in restaurant smoking rooms was confirmed as cotinine levels found in workers' urine. Considering that all workers exhibited normal lung function, eventual molecular changes in blood that might be associated with occupational exposure to SHS and SHS-associated PPAH were investigated by measurement of two oxidative markers, total antioxidant status (TAS) and 8-hydroxyguanosine (8-OHdG) in plasma and serum, respectively. SHS-exposed workers exhibited higher mean levels of serum 8-OHdG than nonexposed workers, regardless of smoking status. By using a proteomics approach based on 2D-DIGE-MS, it was possible to identify nine differentially expressed proteins in the plasma of SHS-exposed nonsmoker workers. Two acute-phase inflammation proteins, ceruloplasmin and inter-alpha-trypsin inhibitor heavy chain 4 (ITIH4), were predominant. These two proteins presented a high number of isoforms modulated by SHS exposure with the high-molecular-weight (high-MW) isoforms decreased in abundance while low-MW isoforms were increased in abundance. Whether these expression profiles are due to (1) a specific proteolytic cleavage, (2) a change on protein stability, or (3) alterations on post-translational modification pattern of these proteins remains to be investigated. Considering that these events seem to precede the first symptoms of tobacco-related diseases, our findings might contribute to elucidation of early SHS-induced pathogenic mechanisms and constitute a useful tool for monitoring the effects of SHS on occupationally exposed individuals such as those working in the hospitality industry.
Peer review: yes
URI: http://hdl.handle.net/10400.18/1735
ISSN: 1528-7394
Versão do Editor: http://www.tandfonline.com/doi/pdf/10.1080/15287394.2013.757269
Aparece nas colecções:DGH - Artigos em revistas internacionais

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