Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.18/1721
Título: Performance of Human Fecal Anaerobe-Associated PCR-Based Assays in a Multi-Laboratory Method Evaluation Study
Autor: Layton, Blythe
Yiping, Cao
Ebentier, Darcy
Kaitlyn, Hanley
Ballesté, Elisenda
Brandão, João
Byappanahalli, Muruleedhara
Converse, Reagan
Farnleitner, Andreas
Gentry-Shields, Jennifer
Gidley, Maribeth
Gourmelon, Michèle
Soo Lee, Chang
Lee, Jiyoung
Lozach, Solen
Madi, Tania
Meijer, Wim
Noble, Rachel
Peed, Lindsay
Reischer, Georg
Rodrigues, Raquel
Rose, Joan
Schriewer, Alexander
Sinigalliano, Chris
Srinivasan, Sangeetha
Stewart, Jill
Van De Werfhorst, Laurie
Wang, Dan
Whitman, Richard
Wuertz, Stefan
Jay, Jenny
Holden, Patricia
Boehm, Alexandria
Shanks, Orin
Griffith, John
Palavras-chave: Microbial Source Tracking
Bacteroidales
qPCR
Water Quality
Bacteroides
Data: 5-Jul-2013
Editora: Elsevier
Citação: Water Res. 2013 Jul 5. pii: S0043-1354(13)00550-2. doi: 10.1016/j.watres.2013.05.060.
Resumo: A number of PCR-based methods for detecting human fecal material in environmental waters have been developed over the past decade, but these methods have rarely received independent comparative testing in large multi-laboratory studies. Here, we evaluated ten of these methods (BacH, BacHum-UCD, B. thetaiotaomicron (BtH), BsteriF1, gyrB, HF183 endpoint, HF183 SYBR, HF183 Taqman, HumM2, and M. smithii nifH (Mnif)) using 64 blind samples prepared in one laboratory. The blind samples contained either one or two fecal sources from human, wastewater or non-human sources. The assay results were assessed for presence/absence of the human markers and also quantitatively while varying the following: 1) classification of samples that were detected but not quantifiable (DNQ) as positive or negative; 2) reference fecal sample concentration unit of measure (such as culturable indicator bacteria, wet mass, total DNA, etc); and 3) human fecal source type (stool, sewage or septage). Assay performance using presence/absence metrics was found to depend on the classification of DNQ samples. The assays that performed best quantitatively varied based on the fecal concentration unit of measure and laboratory protocol. All methods were consistently more sensitive to human stools compared to sewage or septage in both the presence/absence and quantitative analysis. Overall, HF183 Taqman was found to be the most effective marker of human fecal contamination in this California-based study.
Peer review: yes
URI: http://hdl.handle.net/10400.18/1721
ISSN: 0043-1354
Versão do Editor: http://www.sciencedirect.com/science/article/pii/S0043135413005502
Aparece nas colecções:DDI - Artigos em revistas internacionais

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