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Publicação
Tuberculosis diagnosis after bleach processing for early stage tuberculosis laboratory capacity building
Orientador(es)
Resumo(s)
The diagnosis of tuberculosis is seriously hampered in
the absence of standard biosafety laboratory facilities
for specimen concentration and Mycobacterium tuberculosis
culture. Within a laboratory twinning arrangement,
heat-fixed direct smear and sediment from
74 bleach-processed and 20 non-processed specimens
from Cumura Hospital, Guinea-Bissau, were sent to Lisbon
for molecular evaluation of rifampicin resistance.
Sequence analysis of a 369 base-pair rpoB locus detected
3.2% (3/94) resistant specimens. To our knowledge,
this represents the first report on the molecular analysis
of M. tuberculosis from bleach-processed sputum, an alternative
to current diagnostic practice in low-resource
settings.
Descrição
Palavras-chave
Mycobacterium Tuberculosis rpoB Sputum Smear Microscopy Sodium Hypochlorite Denaturing High-performance liquid chromatography (DHPLC) Sequencing Infecções Respiratórias Resistência aos Antimicrobianos
Contexto Educativo
Citação
Int J Tuberc Lung Dis. 2012 Nov;16(11):1535-7. doi: 10.5588/ijtld.11.0658
Editora
International Union Against Tuberculosis and Lung Disease