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Please use this identifier to cite or link to this item: http://hdl.handle.net/10400.18/131

Título: Poly (ADP-ribose) polymerase-1 deficiency does not affect ethylnitrosourea mutagenicity in liver and testis of lacZ transgenic mice
Autor: Louro, Henriqueta
Faustino, Inês
Dias, Anabela
Boavida, Maria G.
Palavras-chave: Parp1
LacZ mice
ENU
Mutant frequency
mutation spectrum
Doenças Genéticas
Issue Date: May-2010
Editora: Wiley
Citação: Environ Mol Mutagen. 2010 May;51(4):322-9. Epub 2010 Mar 1
Resumo: Poly (ADP-ribose) polymerase-1 (Parp1) has been implicated in DNA base excision repair, single- and double-strand break repair pathways, as well as in cell death by apoptosis or necrosis. We used Parp1(-/-) lacZ plasmid-based transgenic mice to investigate whether Parp1 deficiency influences the in vivo mutagenic and clastogenic response to the alkylating agent N-ethyl-N-Nitrosourea (ENU) in somatic and germ-cell tissues. The comparison of the lacZ mutant frequencies (MFs) between Parp1(+/+) and Parp1(-/-) mice showed that the ablation of Parp1 does not affect the spontaneous or ENU-induced MFs in liver and testis. In addition, the spectrum of the ENU-induced mutations was not dependent on the Parp1 status, given that similar spectra, consisting mostly of point mutations and a small fraction of deletions/insertions, wereobserved in organs of both Parp1(-/-) and Parp1(+/+) mice. Sequencing of point mutations revealed a consistent significant increase in A:T --> T:A base substitutions, typically induced by ENU. Overall, we observed that neither the frequency nor the spectrum of ENU-induced mutations demonstrated a specificity that could be attributed to the Parp1 impairment in mice organs. The analysis of micronucleus frequency in peripheral blood reticulocytes showed that ENU was clastogenic in both Parp1(-/-) and Parp1(+/+) mice and had a strong cytotoxic effect in Parp1(-/-) mice only. The present data suggest that, at a whole-organism level, Parp1-independent repair mechanisms may be operative in the removal of ENU-induced DNA lesions or that highly damaged cells may be preferentially committed to death when Parp1 is inactivated.
Arbitragem científica: yes
URI: http://hdl.handle.net/10400.18/131
ISSN: 0893-6692
Versão do Editor: http://onlinelibrary.wiley.com/doi/10.1002/em.20555/pdf
Appears in Collections:DGH - Artigos em revistas internacionais

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