Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.18/1302
Título: Genomic signatures and antiviral drug susceptibility profile of A(H1N1)pdm09
Autor: Giria, Marta
Rebelo-de-Andrade, Helena
Santos, Luís
Martins Correia, Vanessa
Vicente, Sónia
Almeida Santos, Madalena
Palavras-chave: A(H1N1)pdm09
Viral Fitness
Internal Proteins
Antiviral Drug Susceptibility
Risk Assessment
Resistência aos Antimicrobianos
Data: Fev-2012
Editora: Elsevier
Citação: J Clin Virol. 2012 Feb;53(2):140-4. Epub 2011 Dec 15
Resumo: BACKGROUND: Genetic changes in influenza surface and internal genes can alter viral fitness and virulence. Mutation trend analysis and antiviral drug susceptibility profiling of A(H1N1)pdm09 viruses is essential for risk assessment of emergent strains and disease management. OBJECTIVE: To profile genomic signatures and antiviral drug resistance of A(H1N1)pdm09 viruses and to discuss the potential role of mutated residues in human host adaptation and virulence. STUDY DESIGN: A(H1N1)pdm09 viruses circulating in Portugal during pandemic and post-pandemic periods and 2009/2010 season. Viruses were isolated in MDCK-SIAT1 cell culture and subjected to mutation analysis of surface and internal proteins, and to antiviral drug susceptibility profiling. RESULTS: The A(H1N1)pdm09 strains circulating during the epidemic period in Portugal were resistant to amantadine. The majority of the strains were found to be susceptible to oseltamivir and zanamivir, with five outliers to neuraminidase inhibitors (NAIs) identified. Specific mutation patterns were detected within the functional domains of internal proteins PB2, PB1, PA, NP, NS1, M1 and NS2/NEP, which were common to all isolates and also some cluster-specific. DISCUSSION: Modification of viral genome transcription, replication and apoptosis kinetics, changes in antigenicity and antiviral drug susceptibility are known determinants of virulence. We report several point mutations with putative roles in viral fitness and virulence, and discuss their potential to result in more virulent phenotypes. Monitoring of specific mutations and genetic patterns in influenza viral genes is essential for risk assessing emergent strains, disease epidemiology and public health implications.
Peer review: yes
URI: http://hdl.handle.net/10400.18/1302
ISSN: 1386-6532
Versão do Editor: http://www.sciencedirect.com/science/article/pii/S1386653211004537
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