Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.18/1300
Título: Recent developments in the analytical methods for sterols separation in food matrices
Autor: Albuquerque, T.G.
Sanches-Silva, A.
Costa, H.S.
Palavras-chave: Composição de Alimentos
Nutrição Aplicada
Data: 20-Nov-2012
Editora: Instituto Nacional de Saúde Doutor Ricardo Jorge, IP
Resumo: Phytosterols (PS) and the related phytostanols are bioactive compounds of many foodstuffs, especially in food items of plant origin, such as vegetable oils, cereal grains, nuts and seeds. PS are known to have several bioactive properties with various implications on human health, including anti-inflammatory, anti-oxidative, anti-carcinogenic activities and cholesterol lowering capacity. Analytical methods are critical elements for the correct and accurate determination of PS. A review of the last three decades, based on compilation of the available information on the different steps (extraction, saponification, derivatization and chromatographic method) of PS determination was performed. The preparation samples for PS analyses should take into account the nature of matrix, its physical sate, among others. Most of the times, ethanolic or methanolic potassium hydroxide are the selected reagents for the saponification, which can be performed at room temperature or with heating. For derivatization, N,O-Bis (trimethylsilyl) trifluoroacetamide (BSTFA) with 1% of trimethylchlorosilane (TMCS) or N-Methyl-N-(trimethylsilyl) trifluoroacetamide (MSTFA) are the most used reagents. Capillary gas-chromatography (GC), with flame ionization detector (FID) or mass spectrometry (MS), proved to be simple and accurate to perform analytical determinations on this group of compounds. However, high performance liquid chromatography (HPLC) with different detection systems has also been used. Analysis of PS are best carried out using 30 m x 0.25 mm i.d. x 0.25 µm film thickness, flexible, fused capillary columns coated with 5% phenyl and 95% methyl siloxane, because it has a high thermal stability and provides better resolution of peaks. Some of these columns are supplied under the trade designations: DB-5, HP-5, RTx-5 or CP Sil-8. This review aims to contribute for a better understanding of the analytical approaches adopted to determine PS and to follow the tendency over the last few years to enhance the knowledge towards PS content found in food products.
Peer review: yes
URI: http://hdl.handle.net/10400.18/1300
Aparece nas colecções:DAN - Posters/abstracts em congressos internacionais

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