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|Título:||Ultra-high pressure LC for astaxanthin determination in shrimp by-products and active food packaging|
Bernaldo de Quirós, A.
Soto Valdez, H.
Pardo Aurrekoetxea, G.
Ultra-high Pressure Liquid Chromatography
|Editora:||John Wiley and Sons|
|Citação:||Biomed Chromatogr. 2012 Dec 7. doi: 10.1002/bmc.2856. [Epub ahead of print]|
|Resumo:||Nowadays, there is increasing interest in natural antioxidants from food by-products. Astaxanthin is a potent antioxidant and one of the major carotenoids in crustaceans and salmonids. An ultra-high pressure liquid chromatographic method was developed and validated for the determination of astaxanthin in shrimp by-products, and its migration from new packaging materials to food simulants was also studied. The method uses an UPLCW BEH guard-column (2.1 x 5 mm, 1.7mm particle size) and an UPLCW BEH analytical column (2.1 x 50 mm, 1.7mm particle size). Chromatographic separation was achieved using a programmed gradient mobile phase consisting of (A) acetonitrile–methanol (containing 0.05 M ammonium acetate)–dichloromethane (75:20:5, v/v/v) and (B) ultrapure water. This method was evaluated with respect to validation parameters such as linearity, precision, limit of detection, limit of quantification and recovery. Low-density polyethylene films were prepared with different amounts of the lipid fraction of fermented shrimp waste by extrusion, and migration was evaluated into food simulants (isooctane and ethanol 95%, v/v). Migration was not detected under the tested conditions.|
|Versão do Editor:||http://onlinelibrary.wiley.com/doi/10.1002/bmc.2856/abstract;jsessionid=A0024DC54C4EEE9147B7B8AC75933E83.d04t04|
|Aparece nas colecções:||DAN - Artigos em revistas internacionais|
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