Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.18/1283
Título: The plasma membrane-enriched fraction proteome response during adaptation to hydrogen peroxide in Saccharomyces cerevisiae
Autor: Pedroso, Nuno
Gomes Alves, Patricia
Marinho, H. Susana
Brito, Veronica
Boada, Cristina
Antunes, Fernando
Herrero, Enrique
Penque, Deborah
Cyrne, Luísa
Palavras-chave: Proteomics
Genómica Funcional e Estrutural
Eisosomes
Membrane Compartment of Can1p
Oxidative Stress
Plasma Membrane Permeability
Lipid Traffic
Data: Out-2012
Editora: Informa Healthcare
Citação: Free Radic Res. 2012 Oct;46(10):1267-79. Epub 2012 Jul 16
Resumo: n Saccharomyces cerevisiae, adaptation to hydrogen peroxide (H₂O₂) decreases plasma membrane permeability to H₂O₂, changes its lipid composition and reorganizes ergosterol-rich microdomains by a still unknown mechanism. Here we show, by a quantitative analysis of the H₂O₂-induced adaptation effect on the S. cerevisiae plasma membrane-enriched fraction proteome, using two-dimensional gel electrophoresis, that 44 proteins are differentially expressed. Most of these proteins were regulated at a post-transcriptional level. Fourteen of these proteins contain redox-sensitive cysteine residues and nine proteins are associated with lipid and vesicle traffic. In particular, three proteins found in eisosomes and in the eisosome-associated membrane compartment occupied by Can1p were up-regulated (Pil1p, Rfs1p and Pst2p) during adaptation to H₂O₂. Survival studies after exposure to lethal H₂O₂ doses using yeast strains bearing a gene deletion corresponding to proteins associated to lipid and vesicle traffic demonstrated for the first time that down-regulation of Kes1p, Vps4p and Ynl010wp and up-regulation of Atp1 and Atp2 increases resistance to H₂O₂. Moreover, for the pil1Δ strain, H₂O₂ at low levels produces a hormetic effect by increasing proliferation. In conclusion, these data further confirms the plasma membrane as an active cellular site during adaptation to H₂O₂ and shows that proteins involved in lipid and vesicle traffic are important mediators of H₂O₂ adaptation.
Peer review: yes
URI: http://hdl.handle.net/10400.18/1283
ISSN: 1071-5762
Versão do Editor: http://informahealthcare.com/doi/abs/10.3109/10715762.2012.704997
Aparece nas colecções:DGH - Artigos em revistas internacionais



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