Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.18/1282
Título: Signaling Pathways of Proteostasis Network Unraveled by Proteomic Approaches on the Understanding of Misfolded Protein Rescue
Autor: Gomes-Alves, Patricia
Neves, Sofia
Penque, Deborah
Palavras-chave: Cystic Fibrosis
Proteomics
Mass Spectrometry
Genómica Funcional e Estrutural
Proteostasis Network
UPR
ER stress
ERAD
F508del-CFTR
Data: 2011
Editora: Elsevier
Citação: Methods Enzymol. 2011;491:217-33(Chapter thirteen)
Resumo: Attempts to promote normal processing and function of F508del-CFTR, the most common mutant in cystic fibrosis (CF), have been described as potential therapeutic strategies in the management of this disease. Here we described the proteomic approaches, namely two-dimensional electrophoresis (2DE), mass spectrometry (MS), and bioinformatics tools used in our recent studies to gain insight into the proteins potentially involved in lowtemperature or mutagenic treatment-induced rescue process of F508del-CFTR. The proteins identified are part of the proteostasis network, such as the unfolded protein response (UPR) signaling pathways that may regulate the processing of CF transmembrane conductance regulator (CFTR) through the folding and trafficking progression. The complete characterization of these signaling pathways and their regulators in CF will certainly contribute to the development of novel therapeutic strategies against CF.
Peer review: yes
URI: http://hdl.handle.net/10400.18/1282
ISSN: 0076-6879
Versão do Editor: http://www.sciencedirect.com/science/article/pii/B9780123859280000134
Aparece nas colecções:DGH - Capítulos (ou partes) de livros

Ficheiros deste registo:
Ficheiro Descrição TamanhoFormato 
Gomes-Alves_Neves_Penque MEnzymol2011[1].pdf1,32 MBAdobe PDFVer/Abrir    Acesso Restrito. Solicitar cópia ao autor!


FacebookTwitterDeliciousLinkedInDiggGoogle BookmarksMySpace
Formato BibTex MendeleyEndnote Degois 

Todos os registos no repositório estão protegidos por leis de copyright, com todos os direitos reservados.