Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.18/1217
Título: Genotoxic effects of occupational exposure to lead and influence of polymorphisms in genes involved in lead toxicokinetics and in DNA repair
Autor: García-Lestón, Julia
Roma-Torres, Joana
Vilares, Maria
Pinto, Rui
Prista, João
Teixeira, João Paulo
Mayan, Olga
Conde, Joao
Pingarilho, Marte
Gaspar, Jorge Francisco
Pásaro, Eduardo
Méndez, Josefina
Laffon, Blanca
Palavras-chave: Lead
Genotoxicity
T-cell Receptor Mutation Assay
Micronucleus Test
Comet Assay
Genetic Polymorphisms
Ar e Saúde Ocupacional
Genotoxicidade Ambiental
Data: Ago-2012
Editora: Elsevier (Pergamon)
Citação: Environ Int. 2012 Aug;43:29-36. Epub 2012 Mar 30
Resumo: Lead is still widely used in many industrial processes and is very persistent in the environment. Although toxic effects caused by occupational exposure to lead have been extensively studied, there are still conflicting results regarding its genotoxicity. In a previous pilot study we observed some genotoxic effects in a population of lead exposed workers. Thus, we extended our study analysing a larger population, increasing the number of genotoxicity endpoints, and including a set of 20 genetic polymorphisms related to lead toxicokinetics and DNA repair as susceptibility biomarkers. Our population comprised 148 workers from two Portuguese factories and 107 controls. The parameters analysed were: blood lead levels (BLL) and δ-aminolevulinic acid dehydratase (ALAD) activity as exposure biomarkers, and T-cell receptor (TCR) mutation assay, micronucleus (MN) test, comet assay and OGG1-modified comet assay as genotoxicity biomarkers. Lead exposed workers showed markedly higher BLL and lower ALAD activity than the controls, and significant increases of TCR mutation frequency (TCR-Mf), MN rate and DNA damage. Oxidative damage did not experience any significant alteration in the exposed population. Besides, significant influence was observed for VDR rs1544410 polymorphism on BLL; APE1 rs1130409 and LIG4 rs1805388 polymorphisms on TCR-Mf; MUTYH rs3219489, XRCC4 rs28360135 and LIG4 rs1805388 polymorphisms on comet assay parameter; and OGG1 rs1052133 and XRCC4 rs28360135 polymorphisms on oxidative damage. Our results showed genotoxic effects related to occupational lead exposure to levels under the Portuguese regulation limit of 70 μg/dl. Moreover, a significant influence of polymorphisms in genes involved in DNA repair on genotoxicity biomarkers was observed.
Peer review: yes
URI: http://hdl.handle.net/10400.18/1217
ISSN: 0160-4120
Versão do Editor: http://www.sciencedirect.com/science/article/pii/S0160412012000505
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