Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.18/1213
Título: Inter-laboratory variation in DNA damage using a standard comet assay protocol
Autor: Forchhammer, Lykke
Ersson, Clara
Loft, Steffen
Moller, Lennart
Godschalk, Roger W. L.
van Schooten, Frederik J
Jones, George D. D.
Higgins, Jennifer A.
Cooke, Marcus
Mistry, Vilas
Karbaschi, Mahsa
Collins, Andrew R.
Azqueta, Amaya
Phillips, David H.
Sozeri, Osman
Routledge, Michael N.
Nelson-Smith, Kirsty
Riso, Patrizia
Porrini, Marisa
Matullo, Giuseppe
Allione, Alessandra
Stepnik, Maciej
Komorowska, Magdalena
Teixeira, João Paulo
Costa, Solange
Corcuera, Laura-Ana
Lopez de Cerain, Adela
Laffon, Blanca
Valdiglesias, Vanessa
Moller, Peter
Palavras-chave: DNA damage
Comet Assay
Inter-laboratory Variation
Ar e Saúde Ocupacional
Genotoxicidade Ambiental
Data: 2012
Editora: Oxford University Press (United Kingdom Environmental Mutagen Society)
Citação: Mutagenesis. 2012 Nov;27(6):665-72. Epub 2012 Jul 27
Resumo: There are substantial inter-laboratory variations in the levels of DNA damage measured by the comet assay. The aim of this study was to investigate whether adherence to a standard comet assay protocol would reduce inter-laboratory variation in reported values of DNA damage. Fourteen laboratories determined the baseline level of DNA strand breaks (SBs)/alkaline labile sites and formamidopyrimidine DNA glycosylase (FPG)-sensitive sites in coded samples of mononuclear blood cells (MNBCs) from healthy volunteers. There were technical problems in seven laboratories in adopting the standard protocol, which were not related to the level of experience. Therefore, the inter-laboratory variation in DNA damage was only analysed using the results from laboratories that had obtained complete data with the standard comet assay protocol. This analysis showed that the differences between reported levels of DNA SBs/alkaline labile sites in MNBCs were not reduced by applying the standard assay protocol as compared with the laboratory's own protocol. There was large inter-laboratory variation in FPG-sensitive sites by the laboratory-specific protocol and the variation was reduced when the samples were analysed by the standard protocol. The SBs and FPG-sensitive sites were measured in the same experiment, indicating that the large spread in the latter lesions was the main reason for the reduced inter-laboratory variation. However, it remains worrying that half of the participating laboratories obtained poor results using the standard procedure. This study indicates that future comet assay validation trials should take steps to evaluate the implementation of standard procedures in participating laboratories.
Peer review: yes
URI: http://hdl.handle.net/10400.18/1213
ISSN: 0267-8357
Versão do Editor: http://mutage.oxfordjournals.org/content/27/6/665.abstract
Aparece nas colecções:DSA - Artigos em revistas internacionais

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