Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.18/1208
Título: Molecular Epidemiology of Hepatitis A Virus in a Group of Portuguese Citizens Living in Lisbon Area
Autor: Rodrigues, Luisa
Pista, Angela
Oliveira, Ana
Água-Doce, Ivone
Manita, Carla
Paixão, Teresa
Palavras-chave: HAV
VP1-P2a Junction
Phylogenetic Analysis
Sub-genotype IA
Sub-genotype IB
Infecções Sexualmente Transmissíveis
Data: Mai-2007
Editora: Wiley-Liss
Citação: J Med Virol. 2007 May;79(5):483-7
Resumo: Hepatitis A virus (HAV) is the most important cause of acute infectious hepatitis worldwide. In Portugal, due to improvements in sanitation epidemic outbreaks of HAV infection have become less frequent. This report is the first, to our knowledge that characterized HAV in Portugal. For the detection and molecular characterization of HAV cases in a group of Portuguese individuals in the Lisbon area, 31 serum samples were tested: 8 from symptomatic children from an acute hepatitis A outbreak in a Roma (Gipsies) community (2004–2005), and 22 from patients with acuteHAV from sporadic cases (2005–2006). A sample of CSF involved in a case of meningitis was also included. IgM anti-HAV detection and nested reverse transcription (RT-PCR), with primers located at the VP1-P2a region, was undertaken to detect HAV genome. In positive samples, molecular characterization was followed by phylogenetic analysis. All samples (n¼31) were positive for IgM anti-HAV. HAV RNA was found in 96.7% of cases. All isolates were classified as genotype I: 22 belonged to sub-genotype IA (73.3%), and 8 to sub-genotype IB (26.7%). All strains obtained from an acute HAV outbreak had sub-genotype IA, in which seven isolates (87.5%) had identical sequences. In HAV sporadic cases sub-genotypes IA and IB were identified, and this may reflect the co-circulation of these two subgenotypes in Portugal. Molecular epidemiology of HAV infection in this group of Portuguese appears to be similar to other European countries. HAV phylogenetic studies can provide important information for the design of appropriate public health measures.
Peer review: yes
URI: http://hdl.handle.net/10400.18/1208
ISSN: 0146-6615
Versão do Editor: http://onlinelibrary.wiley.com/doi/10.1002/jmv.20851/abstract;jsessionid=FF72AA186C16E31A286C78B8F7C962C9.d04t01
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