Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.18/1178
Título: Fungal and microbial volatile organic compounds exposure assessment in a waste sorting plant
Autor: Malta-Vacas, Joana
Viegas, Susana
Sabino, Raquel
Viegas, Carla
Palavras-chave: Fungi
Molecular Detection
Microbial Volatile Compounds
Solid Waste Sorting
Data: Out-2012
Editora: Taylor and Francis
Citação: J Toxicol Environ Health A. 2012;75(22-23):1410-7
Resumo: In the management of solid waste, pollutants over a wide range are released with different routes of exposure for workers. The potential for synergism among the pollutants raises concerns about potential adverse health effects, and there are still many uncertainties involved in exposure assessment. In this study, conventional (culture-based) and molecular real-time polymerase chain reaction (RTPCR) methodologies were used to assess fungal air contamination in a waste-sorting plant which focused on the presence of three potential pathogenic/toxigenic fungal species: Aspergillus flavus, A. fumigatus, and Stachybotrys chartarum. In addition, microbial volatile organic compounds (MVOC) were measured by photoionization detection. For all analysis, samplings were performed at five different workstations inside the facilities and also outdoors as a reference. Penicillium sp. were the most common species found at all plant locations. Pathogenic/toxigenic species (A. fumigatus and S. chartarum) were detected at two different workstations by RTPCR but not by culture-based techniques. MVOC concentration indoors ranged between 0 and 8.9 ppm (average 5.3 ± 3.16 ppm). Our results illustrated the advantage of combining both conventional and molecular methodologies in fungal exposure assessment. Together with MVOC analyses in indoor air, data obtained allow for a more precise evaluation of potential health risks associated with bioaerosol exposure. Consequently, with this knowledge, strategies may be developed for effective protection of the workers.
Peer review: yes
URI: http://hdl.handle.net/10400.18/1178
ISSN: 1528-7394
Versão do Editor: http://www.tandfonline.com/doi/abs/10.1080/15287394.2012.721175?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%3dpubmed
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