Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.18/1176
Título: Diagnostic Assays for Crimean-Congo hemorrhagic fever
Autor: Vanhomwegen, J.
Alves, M.J.
Zupanc, T.A.
Bino, S.
Chinikar, S.
Karlberg, H.
Korukluoglu, G.
Korva, M.
Mardani, M.
Mirazimi, A.
Mousavi, M.
Papa, A.
Saksida, A.
Sharifi-Mood, B.
Sidira, P.
Tsergouli, K.
Wolfel, R.
Zeller, H.
Dubois, P.
Palavras-chave: Infecções Sistémicas e Zoonoses
Crimean-Congo
Hemorrhagic Fever
Diagnostic Assays
Data: Dez-2012
Editora: CDC-National Center for Infectious Diseases
Citação: Emerg Infect Dis. 2012 Dec;18(12):1958-65
Resumo: Crimean-Congo hemorrhagic fever (CCHF) is a highly contagious viral tick-borne disease with case-fatality rates as high as 50%. We describe a collaborative evaluation of the characteristics, performance, and on-site applicability of serologic and molecular assays for diagnosis of CCHF. We evaluated ELISA, immunofluorescence, quantitative reverse transcription PCR, and low-density macroarray assays for detection of CCHF virus using precharacterized archived patient serum samples. Compared with results of local, in-house methods, test sensitivities were 87.8%-93.9% for IgM serology, 80.4%-86.1% for IgG serology, and 79.6%-83.3% for genome detection. Specificity was excellent for all assays; molecular test results were influenced by patient country of origin. Our findings demonstrate that well-characterized, reliable tools are available for CCHF diagnosis and surveillance. The on-site use of such assays by health laboratories would greatly diminish the time, costs, and risks posed by the handling, packaging, and shipping of highly infectious biologic material.
Peer review: yes
URI: http://hdl.handle.net/10400.18/1176
ISSN: 1080-6040
Versão do Editor: http://wwwnc.cdc.gov/eid/article/18/12/12-0710_article.htm
Aparece nas colecções:DDI - Artigos em revistas internacionais

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Vanhomwegen J Alves MJ et al Diagnostic Assays for CCHF 2012.pdf315,59 kBAdobe PDFVer/Abrir


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