Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.18/1165
Título: Tumour-like phenotypes in urothelial cells after exposure to antigens from eggs of Schistosoma haematobium: an oestrogen-DNA adducts mediated pathway?
Autor: Botelho, Mónica C.
Vale, N.
Gouveia, M.J.
Rinaldi, G.
Santos, J.
Santos, L.L.
Gomes, P.
Brindley, P.J.
Correia da Costa, José Manuel
Palavras-chave: Schistosoma Haematobium
Eggs
HCV29 Cells
Oxidative Stress
Genotoxicity
Oestrogen–DNA Adducts
Bladder Cancer
LC-DAD/ESI-MS
Data: Jan-2013
Editora: Elsevier
Citação: Int J Parasitol. 2013 Jan;43(1):17-26. Epub 2012 Dec 20.
Resumo: Chronic infection with the blood fluke, Schistosoma haematobium, is associated with squamous cell carcinoma of the bladder. Previously, it has been shown that soluble extracts of mixed sex adult S. haematobium worms (SWAP) are tumourigenic, both in vitro and in vivo. In addition, oestrogen-related molecules in SWAP of S. haematobium down-regulate oestrogen receptors (ERs) alpha and beta in oestrogen responsive cells. Moreover, schistosome oestrogens occur in sera of persons with schistosomiasis haematobia and repress transcription of ERs in urothelial cells. Given that eggs of S. haematobium are the developmental stage directly responsible for urogenital disease during schistosomiasis haematobia, we suspected that soluble antigens from S. haematobium eggs exhibit similar or more potent tumorigenic capacity. Here we investigated the tumorigenic potential of soluble egg antigens (Sh-SEA) of S. haematobium and the endocrine system in favouring parasitism by schistosomes. The findings confirmed that 6.25μg/ml of Sh-SEA was enough to stimulate cell proliferation, reduce apoptosis and increase oxidative stress of Sh-SEA-exposed urothelial cells. In addition, genotoxic effects of Sh-SEA on these cells were determined by using alkaline single-cell gel electrophoresis (Comet). Furthermore, Liquid Chromatography Diode Array Detection Electron Spray Ionisation Mass Spectrometry indicated the presence of catechol-oestrogens in S. haematobium SEA. A prospective oestrogen-DNA adduct mediated pathway in S. haematobium egg induced bladder cancer is also discussed.
Peer review: yes
URI: http://hdl.handle.net/10400.18/1165
ISSN: 0020-7519
Versão do Editor: http://www.sciencedirect.com/science/article/pii/S0020751912003104
Aparece nas colecções:DPSPDNT - Artigos em revistas internacionais



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