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|Title: ||Genotypic diversity of clinical Coxiella burnetii isolates from Portugal based on MST and MLVA-typing|
|Authors: ||Santos, A.S.|
|Keywords: ||Coxiella burnetii|
Infecções Sistémicas e Zoonoses
|Issue Date: ||2012|
|Publisher: ||Urban and Fischer / Elsevier|
|Citation: ||Int J Med Microbiol. 2012 Nov;302(6):253-6. Epub 2012 Oct 4|
|Abstract: ||Genotypic diversity of clinical Coxiella burnetii isolates from Portugal based on MST and MLVA typing.
Santos AS, Tilburg JJ, Botelho A, Barahona MJ, Núncio MS, Nabuurs-Franssen MH, Klaassen CH.
SourceCentre for Vectors and Infectious Diseases Research Doutor Francisco Cambournac, National Institute of Health Dr. Ricardo Jorge (CEVDI/INSA), Águas de Moura, Portugal. Electronic address: firstname.lastname@example.org.
The temporal and spatial diversity of Coxiella burnetii genotypes associated with human and animal disease in Portugal was analysed using a 6-locus multiple-locus variable-number tandem repeat analysis (MLVA) and a 10-locus multi-spacer sequence typing (MST) panel. Fifteen cultured C. burnetii isolates from 13 Q fever patients and a stillborn goat and 6 additional PCR-positive ruminant tissue samples obtained during 2006-2011 were included in this study. Seven MLVA genotypes (types S-Y) were obtained, including 4 new MLVA types (U, V, W, and X), all corresponding to 3 MST profiles (types 4, 8, and 13) previously reported from France and Spain. MLVA types U-Y, all belonging to MST type 4, were found in acute Q fever patients from the districts of Évora, Faro, Lisbon, and Setúbal. Different MLVA types were associated with goats from Castelo Branco district (S) and chronic Q fever patients from both Castelo Branco and Lisboa districts (S and T), matching with MST types 13 and 8, respectively. In conclusion, a genotypic diversity of C. burnetii consistent with a non-outbreak situation was identified. The involvement of different genotypes in acute and chronic Q fever was found, linking one of the chronic genotypes to goats from the eastern region of the country.|
|Peer Reviewed: ||yes|
|Publisher version: ||http://www.sciencedirect.com/science/article/pii/S1438422112000549|
|Appears in Collections:||DDI - Artigos em revistas internacionais|
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