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Title: Implication of the RDRio Mycobacterium tuberculosis sublineage in multidrug resistant tuberculosis in Portugal
Author: David, Susana
Duarte, Elsa L.
Leite, Clarice Queico Fugimura
Ribeiro, João-Nuno
Maio, José-Nuno
Paixão, Eleonora
Portugal, Clara
Sancho, Luísa
Germano de Sousa, José
Keywords: Mycobacterium Tuberculosis
Multidrug Resistant Tuberculosis (MDR–TB)
Latin American Mediterranean Family
RDRio Sublineage
Resistência aos Antimicrobianos
Issue Date: 5-May-2012
Publisher: Elsevier
Citation: Infect Genet Evol. 2012 Oct;12(7):1362-7.Epub 2012 May 5
Abstract: Multidrug and extensively drug resistant Mycobacterium tuberculosis are a threat to tuberculosis control programs. Genotyping methods, such as spoligotyping and MIRU–VNTR typing (Mycobacterial Interspersed Repetitive Units), are useful in monitoring potentially epidemic strains and estimating strain phylogenetic lineages and/or genotypic families. M. tuberculosis Latin American Mediterranean (LAM) family is a major worldwide contributor to tuberculosis (TB). LAM specific molecular markers, Ag85C103 single nucleotide polymorphism (SNP) and RDRio long-sequence polymorphism (LSP), were used to characterize spoligotype signatures from 859 patient isolates from Portugal. LAM strains were found responsible for 57.7% of all tuberculosis cases. Strains with the RDRio deletion (referred to as RDRio) were estimated to represent 1/3 of all the strains and over 60% of the multidrug resistant (MDR) strains. The major spoligotype signature SIT20 belonging to the LAM1 RDRio sublineage, represented close to 1/5th of all the strains, over 20% of which were MDR. Analysis of published datasets according to stipulated 12 loci MIRU–VNTR RDRio signatures revealed that 96.3% (129/134) of MDR and extensively drug resistant (XDR) clusters were RDRio. This is the first report associating the LAM RDRio sublineage with MDR. These results are an important contribution to the monitoring of these strains with heightened transmission for future endeavors to arrest MDR–TB and XDR–TB.
Peer review: yes
ISSN: 1567-1348
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Appears in Collections:DEP - Artigos em revistas internacionais
DGH - Artigos em revistas internacionais

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