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Title: First Worldwide Proficiency Study on Variable-Number Tandem-Repeat Typing of Mycobacterium tuberculosis Complex Strains
Authors: de Beer, Jessica
Kremer, Kristin
Ködmön, Csaba
Supply, Philip
van Soolingen, Dick
Global Network for the Molecular Surveillance of Tuberculosis 2009
Keywords: Variable Number Tandem Repeat (VNTR)
Multiple-Locus Variable number tandem repeat Analysis (MLVA)
Quality Control
Tandem Repeats
Resistência aos Antimicrobianos
Issue Date: 14-Dec-2011
Publisher: American Society for Microbiology
Citation: J Clin Microbiol. 2012 Mar;50(3):662-9. Epub 2011 Dec 14
Abstract: Although variable-number tandem-repeat (VNTR) typing has gained recognition as the new standard for the DNA fingerprinting of Mycobacterium tuberculosis complex (MTBC) isolates, external quality control programs have not yet been developed. Therefore, we organized the first multicenter proficiency study on 24-locus VNTR typing. Sets of 30 DNAs of MTBC strains, including 10 duplicate DNA samples, were distributed among 37 participating laboratories in 30 different countries worldwide. Twenty-four laboratories used an in-house-adapted method with fragment sizing by gel electrophoresis or an automated DNA analyzer, nine laboratories used a commercially available kit, and four laboratories used other methods. The intra- and interlaboratory reproducibilities of VNTR typing varied from 0% to 100%, with averages of 72% and 60%, respectively. Twenty of the 37 laboratories failed to amplify particular VNTR loci; if these missing results were ignored, the number of laboratories with 100% interlaboratory reproducibility increased from 1 to 5. The average interlaboratory reproducibility of VNTR typing using a commercial kit was better (88%) than that of in-house-adapted methods using a DNA analyzer (70%) or gel electrophoresis (50%). Eleven laboratories using in-house-adapted manual typing or automated typing scored inter- and intralaboratory reproducibilities of 80% or higher, which suggests that these approaches can be used in a reliable way. In conclusion, this first multicenter study has documented the worldwide quality of VNTR typing of MTBC strains and highlights the importance of international quality control to improve genotyping in the future.
Peer review: yes
ISSN: 0095-1137
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Appears in Collections:DGH - Artigos em revistas internacionais

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