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Please use this identifier to cite or link to this item: http://hdl.handle.net/10400.18/1070

Título: Bridging the gap between PCR detection of Mycobacterium tuberculosis complex and tuberculosis diagnosis
Autor: Duarte, E. L.
Paixão, E.
David, S.
Palavras-chave: Mycobacterium Tuberculosis Complex
Real-time PCR
Acid-fast Smear Microscopy
diagnosis
Tuberculosis
Infecções Respiratórias
Issue Date: 5-Jan-2012
Editora: International Union Against Tuberculosis and Lung Disease
Citação: Int J Tuberc Lung Dis. 2012;16(3):391-3. Epub 2012 Jan 5
Resumo: The growing demand for rapid diagnosis of tuberculosis (TB) has led to the incorporation of nucleic acid amplification (NAA) tests in case definitions. The objective of this study was to evaluate the contribution of a real-time polymerase chain reaction (PCR) assay in providing a result predictive of a confirmed TB case. Respiratory and extra-pulmonary specimens (n = 308) were subjected to NAA, culture and smear microscopy. Qualitative PCR assessment, translated by an increase in NAA cycles, disregarding template copy number, resulted in an increase in confirmed cases, helping to bridge the gap between the test’s analytical performance and its actual performance in TB diagnosis.
Arbitragem científica: yes
URI: http://hdl.handle.net/10400.18/1070
ISSN: 1027-3719
Versão do Editor: http://www.ingentaconnect.com/content/iuatld/ijtld/2012/00000016/00000003/art00021?token=00501aa4aee70266aa573d2570257045497b46423138595e47797c4e75477e4324576b642738eff6
Appears in Collections:DEP - Artigos em revistas internacionais
DGH - Artigos em revistas internacionais

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