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Title: Bridging the gap between PCR detection of Mycobacterium tuberculosis complex and tuberculosis diagnosis
Authors: Duarte, E. L.
Paixão, E.
David, S.
Keywords: Mycobacterium Tuberculosis Complex
Real-time PCR
Acid-fast Smear Microscopy
Infecções Respiratórias
Issue Date: 5-Jan-2012
Publisher: International Union Against Tuberculosis and Lung Disease
Citation: Int J Tuberc Lung Dis. 2012;16(3):391-3. Epub 2012 Jan 5
Abstract: The growing demand for rapid diagnosis of tuberculosis (TB) has led to the incorporation of nucleic acid amplification (NAA) tests in case definitions. The objective of this study was to evaluate the contribution of a real-time polymerase chain reaction (PCR) assay in providing a result predictive of a confirmed TB case. Respiratory and extra-pulmonary specimens (n = 308) were subjected to NAA, culture and smear microscopy. Qualitative PCR assessment, translated by an increase in NAA cycles, disregarding template copy number, resulted in an increase in confirmed cases, helping to bridge the gap between the test’s analytical performance and its actual performance in TB diagnosis.
Peer review: yes
ISSN: 1027-3719
Publisher Version:
Appears in Collections:DEP - Artigos em revistas internacionais
DGH - Artigos em revistas internacionais

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